Viral detection, quantification and characterization

  • End-point dilution quantification and CPE-based antiviral assays (infectious particles) possible for cells supernatant, HAE apical wash, organs and tissues, swabs, etc.
    – Quantification of infectious viral particles by plaque-forming unit assay (PFU)
    – 50% tissue cell infectious dose assay (TCID50)
    – 50% eggs infectious dose assay (EID50)
  • Antigen quantification by Enzyme-linked immunosorbent assay (ELISA) and Enzyme-linked immunospot (ELISpot)
  • PCR, qPCR, RTqPCR of viral and cellular RNA/DNA
  • Viral and cellular RNA/DNA extraction
  • Quantification of viral and cellular nucleic acids
  • Gene expression assay
  • Panviral detection assay (Influenza, RSV, Metapneumovirus, Picornavirus, Adenovirus, Coronavirus, Parainfluenza, MVM Parvovirus, Bocavirus) by molecular biology methods
  • Quantification by the capacity to aggregate red blood cells: hemagglutination assay (HA)
  • Evaluation of influenza neuraminidase enzymatic activity: fluorometric neuraminidase inhibition assay (NAI)
  • Viral antigen quantification: single radial immuno-diffusion assay (SRID)